Pal Maliga

Chloroplast Biotechnology: Methods and Protocols - Methods in Molecular Biology Pal Maliga 2014 edition

Marc Notes: In this volume, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Table of Contents: Part I: Background1. The Plastid Genomes of Flowering PlantsTracey A. Ruhlman and Robert K. Jansen 2. Next Generation Technologies to Determine Plastid Genome Sequences Robert J. Henry, Nicole Rice, Mark Edwards and Catherine J. Nock 3. Plastid Gene Transcription: Promoters and RNA PolymerasesJennifer Ortelt and Gerhard Link 4. Plastid mRNA TranslationMasahiro Sugiura 5. Engineering Chloroplasts for High-Level Foreign Protein ExpressionRalph Bock6. Excision of Plastid Marker Genes Using Directly Repeated DNA SequencesElisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila and Anil Day 7. Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-Green PlastidsMaureen R. Hanson and Amirali Sattarzadeh Part II: Nicotiana Tabacum the Model Species of Chloroplast Biotechnology 8. Plastid Transformation in "Nicotiana tabacum" and "Nicotiana sylvestris "by Biolistic DNA Delivery to LeavesPal Maliga and Tarinee Tungsuchat Huang 9. "Nicotiana tabacum" PEG-mediated Plastid TransformationAreli Herrera Diaz and Hans-Ulrich Koop 10. Plastid Transformation of Tobacco Suspension Cell CulturesJeffrey M. Staub 11. Tryptophan and Indole Analog Mediated Plastid TransformationPierluigi Barone, Xing-Hai Zhang and Jack M. Widholm 12. Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre RecombinaseTarinee Tungsuchat-Huang and Pal Maliga 13. Determination of the Half-Life of Chloroplast Transcripts in Tobacco LeavesSithichoke Tangphatsornruang and John C. Gray 14. Quantification of Organellar DNA and RNA Using Real-Time PCRAndreas Weihe 15. Plastid Transformation for Rubisco Engineering and Protocols for Assessing ExpressionSpencer M. Whitney and Robert E. Sharwood Part III: Crop Specific Plastid Transformation Protocols 16. Plastid Transformation in TomatoStephanie Ruf and Ralph Bock 17. Stable Plastid Transformation of PetuniaElena Martin Avila and Anil Day 18. Plastid Transformation in Potato - "Solanum tuberosum"Vladimir T. Valkov, Daniela Gargano, Nunzia Scotti, and Teodoro Cardi 19. Plastid Transformation in Eggplant Kailash C. Bansal and Ajay K. Singh20. Plastid Transformation in Lettuce ("Lactuca sativa" L.) by Polyethylene Glycol Treatment of ProtoplastsCilia L. C. Lelivelt, Kees M. P. van Dun, C. Bastiaan de Snoo, Matthew S. McCabe, Bridget V. Hogg, and Jacqueline M. Nugent 21. Plastid Transformation in Lettuce ("Lactuca sativa "L)" "by Biolistic DNA DeliveryTracey A. Ruhlman 22. Plastid Transformation in SoybeanManuel Dubald, Ghislaine Tissot and Bernard Pelissier 23. Plastid Transformation in Cabbage ("Brassica oleracea "L. var." capitata "L."") by the Biolistic ProcessMenq-Jiau Tseng, Ming-Te Yang, Wan-Ru Chu, and Cheng-Wei Liu 24. Plastid Transformation in Sugar Beet "Beta vulgaris"Francesca De Marchis and Michele Bellucci 25. Integration and Expression of "gfp" in the Plastid of "Medicago sativa" L. Shaochen Xing, Zhengyi Wei, Yunpeng Wang, Yanzhi Liu, Chunjing Lin Part IV: Plastid Transformation in Algae26. Rapid Screening for the Robust Expression of Recombinant Proteins in Algal PlastidsDaniel Barrera, Javier Gimpel and Stephen Mayfield 27. A Simple, Low Cost Method for Chloroplast Transformation of the Green Alga "Chlamydomonas reinhardtii"Chloe Economou, Thanyanan Wannathong, Joanna Szaub and Saul Purton 28. Tools for Regulated Gene Expression in the Chloroplast of ChlamydomonasJean-David Rochaix, Raymond Surzycki and Silvia Ramundo Part V: Plastid Transformation in Bryophyte29. Plastid Transformation in "Physcomitrella patens"Mamoru Sugita 30. Plastid Transformation of Sporelings and Suspension-cultured Cells from the Liverwort "Marchantia polymorpha "L. Shota Chiyoda, Katsuyuki T. Yamato, and Takayuki Kohchi"Jacket Description/Back: In "Chloroplast Biotechnology: Methods and Protocols, "expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco, and protocols for plastid transformation in crops, and Chlamydomonas and Bryophytes. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, "Chloroplast Biotechnology: Methods and Protocols" seeks to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology, and healthcare."