Lorenza Trabalzini

Ras Signaling: Methods and Protocols - Methods in Molecular Biology Lorenza Trabalzini 2014 edition

Jacket Description/Back: Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, "Ras Signaling: Methods and Protocols" contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging, and omics technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways, and, ultimately, biological activities and functions of RasGTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful "Methods in Molecular Biology" series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, "Ras Signaling: Methods and Protocols" serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of RasGTPases."Table of Contents:1. The Ras Superfamily of Small GTPases: The Unlocked Secrets Luca Goitre, Eliana Trapani, Lorenza Trabalzini, and Saverio Francesco Retta2. A Novel Method for the Production of Fully-Modified K-Ras 4B Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz, and Vadim Gaponenko3. Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, and Mark R. Philips4. Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs) Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam5. Ras GTPases are Both Regulators and Effectors of Redox Agents Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, and Lorenza Trabalzini6. Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell7. Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases Elisa Ferro, Eva Baldini, and Lorenza Trabalzini8. Screening for MAPK Modulators Using an In-Cell Western Assay Simon Schnaiter, Beatrix Furst, Johannes Neu, Frigyes Waczek, Laszlo rfi, Gyorgy Keri, Lukas A. Huber, and Winfried Wunderlich9. Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests Raffaele d Isa, Riccardo Brambilla, and Stefania Fasano10. Functional Phosphoproteomics of Oncogenic KRAS Signaling Putty-Reddy Sudhir and Jeou-Yuan Chen11. Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets Gianni Francesco Guidetti and Mauro Torti12. Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk between Integrins and Cadherins Luca Goitre, Alessandro Morina, and Saverio Francesco Retta13. Fluorescence Microscopy Study of Rap1 Subcellular Localization Luca Goitre, Valentina Cutano, and Saverio Francesco Retta14. AnIn VitroSystem to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1 David F. Kashatus15. Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival Geng-Xian Shi and Catherine N. Kaminski16. Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia17. High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport Lihi Shaulov, Boris Fichtman, and Amnon Harel18. Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox19. Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells Christoph Biskup and Ignacio Rubio20. Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis Camilo Guzman, Maja olman, and Daniel Abankwa21. Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay Audrey Colomba and Anne J. Ridley22. Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy Annica K. B. Gad, Daniel Ronnlund, Jerker Widengren, and Pontus Aspenstrom 23. Yeast as a Model for Ras Signaling Renata Tisi, Fiorella Belotti, and Enzo Martegani24. Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in "Saccharomyces" "cerevisiae" Sonia Colombo and Enzo Martegani25. RAS Proteins Signaling in the Early Metazoan "Dictyostelium discoideum" Enrico Bracco and Barbara Pergolizzi"Publisher Marketing: Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, this book contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioural approaches, advanced high resolution fluorescence and electron microscopy imaging and 'omics' technologies.