John Kuo

Electron Microscopy: Methods and Protocols - Methods in Molecular Biology John Kuo 3rd ed. 2014 edition

Jacket Description/Back: This third edition of "Electron Microscopy: Methods and Protocols" expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryoTEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM, and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and NanoSIMS. Written in the highly successful "Methods in Molecular Biology "series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "Electron Microscopy: Methods and Protocols, Third Edition" provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research."Table of Contents:1. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured CellsJohn J. Bozzola2. Microwave-assisted Processing and Embedding for Transmission Electron MicroscopyPaul Webster 3. Processing Plant Tissues for Ultrastructural Study John Kuo4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and "En Bloc" StainsE. Ann Ellis5. Metal Shadowing for Electron Microscopy Gregory M. Hendricks6. Freeze Fracture and Freeze EtchingDouglas E. Chandler and William P. Sharp7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological SpecimensJohn J. Bozzola8. High-pressure Freezing: Current State and Future ProspectsAndres Kaech and Urs Ziegler9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger ""10. Cryo-Electron Microscopy of Vitreous SectionsPetr Chlanda and Martin Sachse 11. Negative Staining and Cryo-negative Staining: "Applications in Biology and Medicine"J. Robin Harris""12. Electron Microscopy of the Microtubule Cytoskeleton Assembly "Vitro"Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison13. Cryosectioning Fixed and Cryoprotected Biological Material for ImmmunocytochemistryPaul Webster and Alexandre Webster14. Analysis of Specificity in Immunoelectron MicroscopyChristian Hacker and John M. Lucocq15. Cryo-Electron Microscopy of Membrane ProteinsKenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg16. Tracking DNA and RNA Sequences at High ResolutionDu an Cmarko, Anna Ligasova, and Karel Koberna17. Visualization of DNA and Protein-DNA Complexes with Atomic Force MicroscopyYuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko18. Biological Applications of Phase-Contrast Electron MicroscopyKuniaki Nagayama19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses Fei Guo and Wen Jiang20. Electron Tomography for Organelles, Cells and TissuesWanzhong He and Yongning He 21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured CellsShigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Sohl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge24. FIB-SEM Tomography in BiologyCaroline Kizilyaprak25. Correlative Light and Electron Microscopy using Immunolabeled SectionsHeinz Schwarz and Bruno M. Humbel26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological SamplesMiriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEMClarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Hohn, Soufi Nafeey, Paul Walther28. X-Ray Microanalysis in the Scanning Electron MicroscopeGodfried M. Roomans and Anca Dragomir 29. Application of SEM and EDX in Studying Biomineralization in Plant TissuesHonghua He30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Imaging and Cryoscanning Electron MicroscopyYasuhiro Utsumi and Yuzou Sano31. Biological Applications of Energy-Filtered TEMMartin Saunders and Jeremy A. Shaw32. Secondary Ion Mass Spectrometry Imaging of Biological Cells and TissuesNicholas P. Lockyer 33. Elemental and Isotopic Imaging of Biological Samples using NanoSIMSMatt R. Kilburn and Peta L. Clode34. 3D Chemical Mapping: Application of Scanning Transmission (soft) X-ray Microscopy (STXM) in Combination with Angle-Scan Tomography in Bio-, Geo- and Environmental ScienceGregor Schmid and Martin Obst."Review Quotes: From the book reviews: The amount of information in this book is impressive. The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques. (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)"Marc Notes: Preceded by: Electron microscopy / edited by John Kuo. 2nd ed. c2007.; Includes bibliographical references and index. Publisher Marketing: This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.