Fernando Vivanco

Heart Proteomics: Methods and Protocols - Methods in Molecular Biology Fernando Vivanco 2013 edition

Marc Notes: ; 8; This volume offers protocols and updated methods with a particular focus on MS-based methods of protein and peptide quantification and the analysis of posttranslational modifications as well as descriptions of system biology methods.. Table of Contents:1. Large-Scale Characterization of the Murine Cardiac ProteomeJake Cosme, Andrew Emili, and Anthony O. Gramolini 2. Determining Protein Concentrations of the Human Ventricular ProteomeArjen Scholten and Albert J. R. Heck 3. Multidimensional Protein Identification Technology for Direct-Tissue Proteomics of HeartDario Di Silvestre, Francesca Brambilla, and Pier Luigi Mauri 4. Global Protein Quantification of Mouse Heart Tissue Based on the SILAC MouseAnne Konzer, Aaron Ruhs, Thomas Braun, and Marcus Kruger 5. Global Proteomic Profiling and Enrichment Maps of Dilated CardiomyopathyRuth Isserlin, Daniele Merico, and Andrew Emili 6. Characterization of the Human Myocardial Proteome in Dilated Cardiomyopathy by Label-Free Quantitative Shotgun Proteomics of Heart BiopsiesElke Hammer, Katrin Darm, and Uwe Volker 7. Systems Proteomics of Healthy and Diseased ChromatinHaodong Chen, Emma Monte, Thomas M. Vondriska, and Sarah Franklin 8. Proteomic Analysis of Interstitial Aortic Valve Cells Acquiring a Pro-Calcific ProfileMillioni Renato, Elisa Bertacco, Cinzia Franchin, Giorgio Arrigoni, and Marcello Rattazzi 9. Differential Protein Expression Analysis of Degenerative Aortic Stenosis by Itraq LabelingSergio Alonso-Orgaz, Tatiana Martin-Rojas, Enrique Calvo, Juan Antonio Lopez, Fernando Vivanco, and Maria G. Barderas 10. Characterization of Zebrafish Cardiac Proteome Using On Line PH Gradient SCX -RP HPLC MS/MS PlatformJiang Zhang, Kevin A. Lanham, Warren Heideman, Richard E. Peterson, and Lingjun Li 11. Proteomic Analysis of Brain Mitochondrial Proteome and Mitochondrial ComplexesAna Lopez-Campistrous and Carlos Fernandez-Patron 12. Oxidative Modifications of Mitochondria Complex IILiwen Zhang, Patrick T. Kang, Chwen-Lih Chen, Kari B. Green-Church, and Yeong-Renn Chen 13. Detection of O-GLCNAC Modifications on Cardiac Myofilament ProteinsGenaro A. Ramirez-Correa, Isabel Martinez Ferrando, Gerald Hart, and Anne Murphy 14. Quantification of Mitochondrial S-Nitrosylation by CYSTMT6 Switch AssayChristopher I. Murray, Hea Seung Chung, Helge Uhrigshardt, and Jennifer E. Van Eyk 15. Identification of Thioredoxin Target Protein Networks in Cardiac Tissues of a Transgenic MouseCexiong Fu, Tong Liu, Andrew M. Parrott, and Hong Li 16. Using Pure Protein to Build a Multiple Reaction Monitoring Mass Spectrometry Assay for Targeted Detection and QuantitationEric Grote, Qin Fu, Weihua Ji, Xiaoqian Liu, and Jennifer E. Van Eyk 17. A Sequential Extraction Methodology for Cardiac Extracellular Matrix Prior to Proteomics AnalysisJavier Barallobre-Barreiro, Athanasios Didangelos, Xiaoke Yin, Nieves Domenech, and Manuel Mayr 18. Optimized Method for Identification of the Proteomes Secreted by Cardiac Cells Miroslava Stastna and Jennifer E. Van Eyk 19. Secretome of Human Aortic ValvesFernando de la Cuesta, Gloria Alvarez-Llamas, Felix Gil-Dones, Veronica M. Darde, Enrique Calvo, Juan Antonio Lopez, Fernando Vivanco, and Maria G. Barderas 20. A Comparative Study of Immunodepletion and Equalization Methods for Aortic Stenosis Human PlasmaFelix Gil Dones, Veronica M. Darde, Fernando Vivanco, and Maria G. Barderas 15. Identification of Thioredoxin Target Protein Networks in Cardiac Tissues of a Transgenic MouseCexiong Fu, Tong Liu, Andrew M. Parrott, and Hong Li 16. Using Pure Protein to Build a Multiple Reaction Monitoring Mass Spectrometry Assay for Targeted Detection and QuantitationEric Grote, Qin Fu, Weihua Ji, Xiaoqian Liu, and Jennifer E. Van Eyk 17. A Sequential Extraction Methodology for Cardiac Extracellular Matrix Prior to Proteomics AnalysisJavier Barallobre-Barreiro, Athanasios Didangelos, Xiaoke Yin, Nieves Domenech, and Manuel Mayr 18. Optimized Method for Identification of the Proteomes Secreted by Cardiac Cells Miroslava Stastna and Jennifer E. Van Eyk 19. Secretome of Human Aortic ValvesFernando de la Cuesta, Gloria Alvarez-Llamas, Felix Gil-Dones, Veronica M. Darde, Enrique Calvo, Juan Antonio Lopez, Fernando Vivanco, and Maria G. Barderas 20. A Comparative Study of Immunodepletion and Equalization Methods for Aortic Stenosis Human PlasmaFelix Gil Dones, Veronica M. Darde, Fernando Vivanco, and Maria G. Barderas 15. Identification of Thioredoxin Target Protein Networks in Cardiac Tissues of a Transgenic MouseCexiong Fu, Tong Liu, Andrew M. Parrott, and Hong Li 16. Using Pure Protein to Build a Multiple Reaction Monitoring Mass Spectrometry Assay for Targeted Detection and QuantitationEric Grote, Qin Fu, Weihua Ji, Xiaoqian Liu, and Jennifer E. Van Eyk 17. A Sequential Extraction Methodology for Cardiac Extracellular Matrix Prior to Proteomics AnalysisJavier Barallobre-Barreiro, Athanasios Didangelos, Xiaoke Yin, Nieves Domenech, and Manuel Mayr 18. Optimized Method for Identification of the Proteomes Secreted by Cardiac Cells Miroslava Stastna and Jennifer E. Van Eyk 19. Secretome of Human Aortic ValvesFernando de la Cuesta, Gloria Alvarez-Llamas, Felix Gil-Dones, Veronica M. Darde, Enrique Calvo, Juan Antonio Lopez, Fernando Vivanco, and Maria G. Barderas 20. A Comparative Study of Immunodepletion and Equalization Methods for Aortic Stenosis Human PlasmaFelix Gil Dones, Veronica M. Darde, Fernando Vivanco, and Maria G. Barderas"Jacket Description/Back: The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years. Proteomics is a rapidly expanding investigation platform in cardiovascular medicine and is becoming integrated and incorporated into cardiovascular research. The proteomics field continues to develop with major improvements in mass spectrometry instrumentation, methodology and data analysis. "Heart Proteomics: Methods and Protocols" complies a selection of techniques and methods that target the numerous processes implicated in the pathophysiology of heart. Chapters cover protocols and updated methods in the heart proteomic area with a particular focus on MS-based methods of protein and peptide quantification and the analysis of posttranslational modifications as well as descriptions of system biology approaches, which provide a better understanding of normal and pathological processes. Written in the successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, "Heart Proteomics: Methods and Protocols" is a representative selection of methods that will prove to be a useful resource for experienced proteomics practitioners and newcomers alike. "