Biji T Kurien

Detection of Blotted Proteins: Methods and Protocols - Methods in Molecular Biology Biji T Kurien 2015 edition

Jacket Description/Back: This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the "Methods in Molecular Biology" series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, "Detection of Blotted Proteins: Methods and Protocols" presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. "Table of Contents:1. Western Blotting: Origin and Ascent of the SpeciesW. Neal Burnette 2. Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic EvaluationJ. P. Dean Goldring 3. Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to NitrocelluloseJ. P. Dean Goldring 4. Detection of Blotted Proteins: Not all Blockers are Created EqualVishal Kothari and Suresh T. Mathews 5. Protein Stains to Detect Antigen on MembranesAnil D souza and Hal Scofield 6. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western BlottingF. Javier Alba and Salvador Bartolome 7. Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment ComplementationNeil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, and Keith R. Olson 8. Use of Non-Radioactive Detection Method for North- and South-Western BlotClaudia Franke, Daniel Grafe, Holger Bartsch, Michael Bachmann 9. Immunoblotting using Radiolabeled Reagents for DetectionHolger Bartsch, Claudia Franke, Michael Bachmann 10. Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence TechniqueYaser Dorri 11. Detection of Protein Carbonyls by Means of Biotin Hydrazide Streptavidin Affinity MethodsKenneth Hensley 12. Direct Immunodetection of Antigens within the Precast Polyacrylamide GelSurbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend 13. Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence AssaysVince Boveia and Amy Schutz-Geschwender14. Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots Fangmao Ye, Polina B Smith, and Daniel T. Chiu 15. Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics WorkflowCaitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, and Michael J. Dunn 16. Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and ImmunohistochemistryThomas Millar, Ronald Knighton and Jo-Anne Chuck 17. Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane Biji T. Kurien and Michael Bachmann 18. Sequential use of Immunoblots for Characterization of Autoantibody SpecificitiesHolger Bartsch and Michael Bachmann 19. Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western BlottingJacen S. Moore and R. Hal Scofield 20. Application of Intermittent Microwave Irradiation to Western Blot AnalysisYu-Ting Liu and Shinya Toyokuni 21. Visualization of Unstained Protein Bands on PVDFJun Park, Masaharu Mabuchi, and Ajay Sharma 22. Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon(r)-FL MembraneJun Park, Masaharu Mabuchi, and Ajay Sharma 23. Cold Microwave-Enabled Protein Detection and QuantificationNiels Grutzner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jorg M. Steiner, and Andreas K. Holzenburg 24. TLC-Blot (Far-Eastern Blot) and Its Application to Functional LipidomicsTakao Taki 25. Analysis of Electroblotted Proteins by Mass SpectrometryJose L. Luque-Garcia and Thomas A. Neubert 26. On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium IonsBiji T. Kurien and Michael P. Bachmann 27. Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific FluorophoreLee Broderick Bockus and R. Hal Scofield 28. Purification of Tryptic Digests on Polyvinylidene Difluoride MembraneBiji T. Kurien and R. Hal Scofield 29. Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by AltaJayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole 30. Non-stripping Rainbow and Multiple Antigen Detection (MAD) Western BlottingStan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski 31. Supported Molecular Matrix ElectrophoresisYu-ki Matsuno and Akihiko Kameyama 32. Parafilm-M(r), an Available Cost Effective Alternative for Immuno-blot Pouches Syed MS Quadri 33. Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride MembranesAkihiko Kameyama, Weijie Dong, and Yu-ki Matsuno 34. Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in SalivaSuresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari, M. Pharm 35. A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMBParmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan, and Madan Lal Bhatt, Monika Sachdev 36. Other Notable Methods of Membrane Protein Detection: A Brief ReviewBiji T. Kurien and R. Hal Scofield 37. Nitrocellulose Membrane: The New CanvasJasmin R. Kurien and Bianca A Kurien 38. Invisible Ink Marking in ECL Membrane AssaysBiji T. Kurien "