Valentin Kohler

Protein Design: Methods and Applications - Methods in Molecular Biology Valentin Kohler 2nd Ed. 2014 edition

Jacket Description/Back:"Protein Design: Method and Applications, Second Edition"expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters onmetals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful"Methods in Molecular Biology"series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, """Protein Design: Method and Applications, Second Edition"provides a number of practical protocols and instructive reviews to aid in the creation of new experiments."Table of Contents:1. De novo Design of Stable -helicesAlexander Yakimov, Georgy Rychkov, and Michael Petukhov 2. Design of Monomeric Water-soluble b-hairpin and -sheet PeptidesM. Angeles Jimenez 3. Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-forming Peptides Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, and Anna Mitraki 4. Posttranslational Incorporation of Non-canonical Amino Acids in the RNaseS System by Semisynthetic Protein AssemblyMaika Genz and Norbert Strater 5. Design, Synthesis, and Study of Fluorinated ProteinsBenjamin C. Buer and E. Neil G. Marsh 6. High-Quality Combinatorial Protein Libraries Using the Binary Patterning ApproachLuke H. Bradley 7. Methods for Library-Scale Computational Protein DesignLucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow 8. Symmetric Protein Architecture in Protein Design: Top-Down Symmetric DeconstructionLiam M. Longo and Michael Blaber 9. Identification of Protein Scaffolds for Enzyme Design using Scaffold SelectionAndre C. Stiel, Kaspar Feldmeier, and Birte Hocker 10. Computational Design of Novel Enzymes without CofactorsMatthew D. Smith, Alexandre Zanghellini, and Daniela Grabs 11. De novo Design of Peptide Scaffolds as Novel Pre-organised Ligands for Metal-ion CoordinationAimee J. Gamble and Anna F. A. Peacock 12. Computational Design of MetalloproteinsAvanish S. Parmar, Douglas Pike, and Vikas Nanda 13. Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein ScaffoldsKoji Oohor and Takashi Hayashi 14. Computational Redesign of Metalloenzymes for Catalyzing New ReactionsPer Jr. Greisen and Sagar D. Khare"Marc Notes: Preceded by Protein design / editied by Raphael Guerois, Manuela Lopez de la Paz. c2006."