Gaetano Vitale

Regulatory B Cells: Methods and Protocols - Methods in Molecular Biology Gaetano Vitale

Jacket Description/Back:"Regulatory B Cells: Methods and Protocols" presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, "Regulatory B Cells: Methods and Protocols" serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology."Table of Contents: Part I: Purification and Phenotypic Characterization of B Cell Subsets 1. Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti 2. Purification and Immune-Phenotyping of B-1 Cells from Body Cavities of Mice Vanessa Yenson and Nicole Baumgarth 3. Purification and Immunophenotypic Characterization of Murine B10 B Cells Chao Hong and Xiao-Ming Gao 4. Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions Jean-David Bouaziz, Helene Le Buanec, and Adele de Masson Part II: Mechanisms of Immune Suppression by B Cells 5. IL-10 Detection in Murine B Cells: Pros and Cons of the Different Techniques Jun Deng, Xiang Lin, and Liwei Lu 6. TGF- Detection and Measurement in Murine B Cells: Pros and Cons of the Different Techniques Yoshiyuki Mishima, Shunji Ishihara, Jonathan J. Hansen, and Yoshikazu Kinoshita 7. Characterization and Activity of Fas Ligand Producing CD5+ B Cells Steven K. Lundy and Matthew W. Klinker Part III: Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells 8. Utilization of a Lentiviral System for the Generation of B Cells with Regulatory Properties Elisabeth Calderon-Gomez and Simon Fillatreau 9. Ex Vivo Generation of Murine IL-10-Producing B Cells by Fusokines Aurelie Tormo, Jiusheng Deng, Edouard Al-Chami, Ziouani Sonia, and Moutih Rafei 10. Toll-Like Receptor Ligation for the Induction of Regulatory B Cells Lucien E. P. M van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E. D Martha, and Hermelijn H. Smits 11. The Generation of Regulatory B Cells by Helminth Parasites Adnan Khan, Sylvie Amu, Sean P. Saunders, and Padraic G. Fallon 12. Use of Co-Cultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions Francesca Mion, Federica Benvenuti, Carlo Pucillo, and Gaetano Vitale Part IV: Study of Regulatory B Cells in Pathological Settings 13. Regulatory B Cells in Experimental Mouse Models of Arthritis Elizabeth C. Rosser and Claudia Mauri 14. Regulatory B Cells in Mouse Models of Systemic Lupus Erythematosus (SLE) Takashi Matsushita 15. Regulatory B Cells in Allergic Airways Disease and Asthma Prabitha Natarajan, Linda A. Guernsey, and Craig M. Schramm 16. Regulatory B Cells in Mouse Models of Intestinal Inflammation Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, and Atsushi Mizoguchi 17. Regulatory B Cells in Experimental Autoimmune Encephalomyelitis (EAE) Avijit Ray and Sreemanti Basu 18. Regulatory B Cells, Helminths, and Multiple Sclerosis Jorge Correale and Tomas Rivero Equiza 19. Generation and Identification of Tumor-Evoked Regulatory B Cells Arya Biragyn, Catalina Lee-Chang, and Monica Bodogai"Marc Notes: Includes bibliographical references and index. Publisher Marketing:"Regulatory B Cells: Methods and Protocols" present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, "Regulatory B Cells: Methods and Protocols" serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.