Elizabeth F Hounsell

Glycoprotein Analysis in Biomedicine - Methods in Molecular Biology Elizabeth F Hounsell 1993 edition

Marc Notes: Includes bibliographical references and index. Review Quotes: ...cover(s) a wide range of potential interests...indispensable for anyone starting...as well as...a good many experienced workers. - The AnalystTable of Contents: A General Strategy for Glycoprotein Oligosaccharide Analysis. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography. Sequence and Linkage Analysis of N- and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry. Gas Chromatography and Gas Chromatography-Mass Spectrometry of Glycoprotein Oligosaccharides. Microscale Sequencing of N-Linked Oligosaccharides of Glycoproteins Using Hydrazinolysis, Bio-Gel P-4, and Sequential Exoglyclosidase Digestion. Analysis of Sugar Chains by Pyridylamination. Analysis of Carbohydrates in Glycoproteins by High-Performance Liquid Chromatography and High-Performance Capillary Electrophoresis. The Microanalysis of Glyocosyl-Phosphatidylinositol Glycans. Analysis of Bacterial Glycoproteins. Isolation and Fractionation of Glycoprotein Glycans in Small Amounts. Immunological Detection of Glycoproteins on Blots Based on Labeling with Digoxigenin. Glycosyltransferases as Tools in Cell Biological Studies. Purification of the EGF Receptor for Oligosaccharide Studies. Analysis of Sulfated Polysaccharide Conjugates. Secreted Mucus Glycoproteins in Cell and Organ Culture. Preparation of Polyclonal Antibodies to Native and Modified Mucin Antigens. The Application of Lectins to the Study of Mucosal Glycoproteins. Detection of Tumor-Associated Expression of Carbohydrate-Binding Proteins (Lectins): The Use of Neoglycoproteins and Neoglycoenzymes in Glycohistochemical and Clycocytological Studies. Scanning Tunneling Microscopy and Biopolymers. Glossary. Index. Publisher Marketing: This is a collection of detailed protocols designed to make carbohydrate biochemistry accessible to a wider audience.

Contributor Bio:  Hounsell, Elizabeth F Hounsell, University College London, London, UK.